Guide de culture BudTrainer

CO2 for a Grow Tent: When It Works and When It Is Wasted

A grow tent with its door open, flowering cannabis in black fabric pots under an LED bar light, and beside it an aluminum CO2 cylinder chained to a concrete wall with a brass regulator, gauge, flow meter and solenoid valve feeding a tube into the tent.

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By Henrique Dias, CEO & Co-founder of BudTrainer
Last updated October 2026 · 28 min read

Quick answer: CO2 works on cannabis. In Mitchell Westmoreland's growth-chamber trials in Bruce Bugbee's lab at Utah State University, raising CO2 from outdoor air (about 420 parts per million, ppm) to 1,400 ppm raised flower yield by 43%, and the gain was the same under moderate light as under very bright light.1 But almost no home grow tent can use it. A tent with an exhaust fan replaces its air every minute or so, and at 1,200 ppm that fan carries away 45 to 120 times more CO2 than the plants take in: of every gram you add, 1 to 2 percent reaches a leaf, and a 20 lb (9 kg) cylinder lasts 13 to 34 hours of lights-on. CO2 pays in a sealed room with an air conditioner and a dehumidifier doing the exhaust fan's job. In an ordinary vented tent, the CO2 job is the opposite one: keep the air from falling below room level, because in the same research's yield model a 100 ppm shortfall cost more (15%) than a 100 ppm boost added (10%).
A 4 x 4 ft grow tent with its door rolled up, a flat canopy of flowering cannabis plants in black fabric pots under an LED bar light, and beside it an aluminum CO2 cylinder chained to a basement wall, with a brass regulator, gauge, flow meter tube and solenoid valve and a thin black tube running into the tent.

Most guides to CO2 for a grow tent are shopping lists ranked by price. They skip the only question that matters: where the CO2 goes after you release it. A grow tent is a box with a fan on top pulling room air through it, and the fan does not care what is in that air.

This guide does that arithmetic: what CO2 does and what the research measured, where it goes in a vented tent, why your exhaust fan is your most important CO2 equipment, every way to add CO2 compared by what it can deliver, a seven-step sealed-room setup, and how to stay safe around an invisible gas. If heat is what is limiting your tent rather than CO2, our grow tent temperature guide is the place to start.

The Short Answer: Does a Grow Tent Need CO2?

It depends on whether your grow space trades air with the rest of the house. Find your setup below.

Your setup Add CO2? What to do instead, or as well
A tent with an exhaust fan running most of the lights-on period (most home tents) No. It goes out the duct. Keep the exhaust running so the tent stays at room CO2, spend the money on light and an even canopy
A tent with a small, slow or passive exhaust No Check for depletion first: a weak exhaust can leave the tent 100 ppm or more below room air all day
A tent inside a closed room that has its own air conditioner and dehumidifier Yes, enrich the room Let the tent exhaust into the room, not outdoors, and run CO2 on a controller with a canopy sensor
A sealed grow room, no air exchanged with outside while the lights are on Yes A cylinder and regulator on a controller, 800 to 1,200 ppm, lights-on only
Seedlings, clones and mother plants Not worth it Small plants need little; fresh air is plenty

The target, when you can hold one, is 800 to 1,200 ppm while the lights are on. In Westmoreland's trials, going from 420 to 800 ppm delivered about 70% of the total yield gain, and 1,200 ppm delivered 90% of it.1 Above 1,200 ppm you are paying for the last tenth.

A small converted grow room seen through its doorway: flowering cannabis plants in black fabric pots under an LED fixture, a white ductless mini-split air conditioner high on the wall, a white dehumidifier with a drain hose on the floor, and an aluminum CO2 cylinder chained in the corner with a thin tube running up toward the ceiling.

What CO2 Does for a Cannabis Plant

A plant builds itself out of air. Light supplies the energy, the roots supply water, and carbon dioxide (CO2) comes in from the air through tiny pores on the underside of the leaf, called stomata. Inside the leaf, photosynthesis turns the CO2 and water into sugar, and the sugar becomes stems, roots and buds. Every bit of carbon in a dried flower was CO2 in the air of your tent a few weeks earlier.

Illustration titled What CO2 does inside a leaf: a cannabis fan leaf lit from above, with small air bubbles entering it, water rising up the leaf stalk and sugar flowing down, labeled light, CO2 from the air through tiny pores, water from the roots and sugar sent to the roots and buds.

Outdoor air holds about 425 parts per million (ppm) of CO2: 425 molecules of CO2 in every million molecules of air. NOAA's Mauna Loa record put the 2024 average at 424.6 ppm and the preliminary 2025 average at 427.4.2 That is far less than a cannabis leaf can use. The enzyme that grabs CO2 inside the leaf (rubisco) works faster with more CO2 around it and wastes less energy grabbing oxygen by mistake, so photosynthesis climbs as CO2 rises, well past outdoor levels.

At the other end, a cannabis leaf stops taking in CO2 altogether at about 50 ppm. Westmoreland measured that floor, the CO2 compensation point, at 50 ppm, give or take 5.1 Between 50 and 425 ppm, every ppm the plant loses costs it growth.

A leaf reading is not a yield

The best-known cannabis CO2 numbers came from Suman Chandra, Hemant Lata, Ikhlas Khan and Mahmoud ElSohly at the University of Mississippi. They clamped single leaves into a measuring chamber and found that raising CO2 from 350 to 750 ppm raised photosynthesis by 50% and more than doubled water use efficiency, while dropping CO2 to 250 ppm cut photosynthesis by half.3 A follow-up on four high-THC varieties found that 700 ppm raised photosynthesis significantly over 390 ppm, while 545 ppm made no significant difference in most cases.4 A modest bump does little.

Those are short readings on single leaves, taken in minutes. A whole plant adjusts over weeks. Elizabeth Ainsworth and Alistair Rogers pooled the big outdoor CO2 trials and found that light-saturated photosynthesis in plants like cannabis rose by 31% under long-term elevated CO2, less than short-term readings predict, because the leaves turn down part of their CO2-fixing machinery once CO2 is plentiful.5 Westmoreland saw the same thing in cannabis: the short-term gain in leaf and canopy photosynthesis was bigger than the long-term gain in yield.1 So the number to plan around is a yield number, and until recently cannabis did not have one.

What the Research Actually Says About CO2 and Cannabis Yield

The first controlled yield measurement we know of is Westmoreland's 2024 PhD research at Utah State University, supervised by Bruce Bugbee.1 He grew two high-CBD cultivars, Luck and Peach, in 1.4 m² (15 sq ft) growth chambers for eight weeks of flowering. One study held CO2 at 480, 1,000 and 1,400 ppm under two light levels, 750 and 1,500 µmol/m²/s (micromoles of light per square meter per second, the PPFD a light meter reads), which over a 12-hour day is a daily light integral of 32 and 65 mol/m²/day. Our DLI calculator does that sum for any light and schedule. The second held 460, 800 and 1,200 ppm under 1,000 µmol/m²/s. CO2 came from compressed liquid CO2, it was measured continuously with a laboratory gas analyzer, and it was not enriched at night.

Line chart titled CO2 and cannabis flower yield, from Westmoreland's growth-chamber trials at Utah State, 2024: yield relative to outdoor air at 420 ppm falls 15% at 320 ppm and 40% at 220 ppm, and rises 10% at 520, 20% at 620, 30% at 800, 39% at 1,200 and 43% at 1,400 ppm, with a target band shaded from 800 to 1,200 ppm.

What he found:

  • Yield rose 43% from 420 to 1,400 ppm, and above-ground biomass 39%. In the second study, going from 460 to 1,200 ppm raised yield 34%.
  • The gain was the same at moderate and very bright light. The relative increase held across cultivars and across a daily light integral from 32 to 65 mol/m²/day. Doubling the light doubled yield on its own, and CO2 added its 40-odd percent on top.
  • Most of the gain comes early. 420 to 800 ppm accounted for about 70% of the yield increase, and 90% of it was in hand at 1,200 ppm.
  • Cannabinoid concentration did not change consistently. It fell about 25% in one treatment and was unaffected in the rest. CO2 is a yield tool, not a potency tool.
  • Falling below room air costs more than rising above it gains. His model put the loss from 420 down to 320 ppm at 15% and from 420 to 220 ppm at 40%, while 100 ppm above ambient added 10% and 200 ppm added 20%.
  • Buds ran warmer and leaves took up less calcium and magnesium. Flowers sat 2 to 5°C (4 to 9°F) above the air temperature, and raising CO2 from 480 to 1,400 ppm added about 2°C (3.6°F) more. Calcium and magnesium in leaves and flowers fell about 10%. He fed a double-strength solution as needed to keep the runoff at an EC of 1.0 mS/cm (EC measures how much fertilizer is dissolved in the water), because plants at 1,200 ppm use less water per gram of growth and so take in less of whatever the water carries.

Two caveats keep this honest. It is one lab, two high-CBD cultivars and a dissertation rather than a journal paper, so treat 43% as the best number available, not a law. And it was measured in an ideal environment, which is exactly the point: Westmoreland concluded the yield benefit of elevated CO2 is about 40% across crops like cannabis "when other environmental factors are non-limiting". His figure also lines up with the broad record. Bruce Kimball's 1983 review of 430 observations across 37 crops predicted a 33% yield increase for a doubling of CO2.6

One more thing it settles. You will read that CO2 only helps above 1,000 µmol/m²/s of light, so a home light is too weak for it. Westmoreland's gain was the same at 750 µmol/m²/s as at 1,500. Our grow light distance guide lists the light targets by stage. Light and CO2 stack. And light keeps paying without any CO2 at all: in Victoria Rodriguez-Morrison, David Llewellyn and Youbin Zheng's trial at the University of Guelph, run at ordinary room CO2 (437 ppm with the lights on), yield rose in a straight line all the way to 1,800 µmol/m²/s.7 The obstacle to CO2 in a home grow is not the light. It is the air.

Where the CO2 in a Vented Tent Actually Goes

Take a 4 ft by 4 ft (120 x 120 cm) tent, 6.5 ft (200 cm) tall, flowering under a 480 W LED. Our temperature guide sizes the exhaust for heat: about 0.63 CFM (cubic feet per minute) of real airflow per watt, so about 302 CFM for this light. The popular rule of one tent volume a minute gives about 112 CFM. Either way, the air in the tent is replaced every 20 seconds to a minute.

To hold that tent at 1,200 ppm while the fan pulls in room air at 425 ppm, every cubic foot that leaves takes 775 ppm of your CO2 with it. CO2 weighs about 1.8 grams per liter at 77°F (25°C), so:

  • At 112 CFM the exhaust carries away about 265 g (9.3 oz) of CO2 an hour.
  • At 302 CFM it carries away about 715 g (25 oz) an hour.
  • The plants themselves take in about 6 g (0.2 oz) an hour. That is our estimate for a dense flowering canopy filling 1.5 m² (16 sq ft), taking in 25 µmol of CO2 per square meter of floor per second. Double it and nothing below changes.
Bar chart titled Where the CO2 goes in a vented tent, holding a 4 x 4 ft tent at 1,200 ppm: the plants take in about 6 g an hour, while the exhaust carries away 265 g an hour at 112 CFM and 715 g an hour at 302 CFM, so 1 to 2 percent reaches a leaf and a 20 lb cylinder lasts about 34 or 13 hours of lights-on.

So the fan removes 45 to 120 times what the plants use, and 1 to 2 percent of the CO2 you pay for ever reaches a leaf. A 20 lb (9 kg) cylinder, the common size at a welding or beverage gas supplier, lasts about 34 hours of lights-on at 112 CFM and about 13 hours at 302 CFM. In a 12/12 flowering room that is one to three days per cylinder, for eight to ten weeks.

This is why CO2 kit instructions often tell you to switch the exhaust off while the CO2 is on. That works for CO2 and breaks everything else, because the exhaust was the thing removing the heat from the light and the water from the plants. A 480 W light in a closed tent is a 480 W heater in a closed box; our temperature guide works through why a tent's temperature is its intake air plus a rise set by the watts and the airflow, and with the airflow at zero the rise has no ceiling. The plants also put most of the water you pour back into the tent air, which our grow tent humidity guide puts at about 5.6 pints (2.6 L) a day per plant in a 5 gallon (19 L) pot in mid flower. Turn the fan off and something else has to take that heat and water out.

The half measure: CO2 only while the fan rests

Some controllers inject CO2 only while the exhaust is idle and lock it out when the fan starts. It wastes less, but in a typical LED tent in flower the fan runs most of the lights-on period and flushes the enriched air in under a minute each time it starts. It is a way to waste less, not a way to hold 1,200 ppm.

Your Exhaust Fan Is Already Your Most Important CO2 Equipment

Turn the problem around. A tent of flowering plants under a strong light eats CO2 quickly, and the only thing refilling it is the air your exhaust pulls in. Our model of the same 4 ft by 4 ft (120 x 120 cm) tent, with no CO2 added, shows how fast that matters:

  • Sealed, no air in or out: the canopy pulls the tent from 425 ppm to about 300 ppm in under 10 minutes and under 200 ppm in about 20.
  • A 10 CFM trickle, a passive vent or a very small fan: the tent settles near 300 ppm and stays there all day.
  • 40 CFM: about 380 ppm, 45 below the room.
  • 112 CFM, one tent volume a minute: about 410 ppm, within about 15 ppm of the room.
Line chart titled How fast flowering plants empty a closed tent: after lights-on, a sealed 4 x 4 ft tent falls from 425 ppm to about 70 ppm within an hour, a 10 CFM trickle settles near 300 ppm, 40 CFM near 380 ppm and 112 CFM near 410 ppm, with a line at 50 ppm where the leaf can no longer take in CO2.

Put those against Westmoreland's model and the slow-exhaust tent is the expensive one. A tent stuck near 300 ppm is about 125 ppm below room air, and his model puts a 100 ppm shortfall at 15% of yield. That is a bigger number than most growers would ever get from adding CO2 to a vented tent, and fixing it costs a correctly sized fan.

Illustration titled The exhaust fan is your CO2 supply: on the left a grow tent with its exhaust duct and a low intake, full of air bubbles, labeled exhaust running, stays within about 15 ppm of the room; on the right the same tent closed, with few bubbles left, labeled sealed, nothing added, falls from 425 to under 200 ppm in about 20 minutes.

The model is ours, with stated assumptions: room air at 425 ppm, the canopy taking in 25 µmol/m²/s at room CO2 and less as CO2 falls, down to nothing at 50 ppm. Real tents leak through zippers and seams, so a "sealed" tent falls somewhat slower. The shape does not change: a tent that does not exchange its air runs short of CO2 within minutes of lights-on. Greenhouse growers know the same effect from winter, when the vents stay shut to hold heat: Westmoreland notes that CO2 in a closed greenhouse can fall more than 200 ppm below outdoor air.

The top of a grow tent with silver walls: a black carbon filter and an inline exhaust fan joined by silver flexible duct, hanging from rope hangers above an LED bar light and young cannabis plants.

Three things keep a vented tent at room level:

  • Run the exhaust through the whole lights-on period, sized for heat as worked out above. Do not put it on a timer that stops it for long stretches with the lights on.
  • Give it a real intake. A tent with every port zipped shut makes the fan pull through the seams. Open a low passive vent or run an intake duct from a cool, airy part of the house.
  • Move air inside the canopy. A dense canopy holds still air between its leaves, and that pocket runs low on CO2 even when the tent air above it is fine. A small fan at canopy height keeps it mixed.
A small black oscillating fan clamped to a vertical pole at canopy height inside a silver grow tent, blowing across a flat canopy of flowering cannabis plants in black fabric pots.

Inside the canopy is where it shows first. Air moves freely over the tops, but between dense leaves it sits still, and the leaves there use up the CO2 around them faster than it diffuses back in.

Diagram titled Air Exchange Above and Inside a Dense Canopy: air with CO2 mixes freely above a dense cannabis canopy, labeled free mixing, room level, while the air inside the canopy is labeled still air, locally depleted.

The same goes for the room the tent breathes from. A small closed room with people in it can sit well above outdoor CO2, and a tent in a well ventilated basement will sit near outdoor levels. A cheap CO2 monitor in the room tells you which you have.

A small white CO2 monitor with a blank screen sitting on a wooden bench in a basement beside a black grow tent with a silver duct leaving its top.

When CO2 Enrichment Is Worth It

Enrichment pays when the CO2 you add stays around the plants for hours, not seconds. You need every line of this list.

  • The space is closed while the lights are on. A sealed grow room, or a tent that exhausts into a closed room rather than to the outdoors or the rest of the house.
  • An air conditioner removes the heat. Sized from the plug watts, as our air conditioner sizing section shows. A dual-hose portable, a window unit or a ductless mini-split.
  • A dehumidifier removes the water, sized to the water you pour, as in our humidity guide.
  • Everything else is already right. An even canopy under enough light, the right feed and pH, no pests. CO2 multiplies what you have; it does not fix a canopy with three tall colas and twenty shaded sites. Nor does it license commercial tricks: our defoliation guide explains why the heavy leaf stripping some CO2-enriched commercial rooms use costs a home grow yield.
  • A controller with a sensor, not a timer, decides when the gas flows.
Cut-away illustration titled What CO2 enrichment needs: a sealed room, with flowering cannabis plants under a light, a CO2 cylinder and regulator in the corner, a sensor hanging at the canopy, a wall air conditioner, a dehumidifier on the floor and a closed door, and no exhaust duct.

With those in place the economics flip. Take a 10 ft by 10 ft (3 x 3 m) room, 8 ft (2.4 m) tall, holding one 4 ft by 4 ft tent's worth of flowering plants. A closed room in a house still leaks; at half an air change an hour, meaning half the room's air leaks out every hour (our assumption; a closed interior room can be tighter), holding 1,200 ppm loses about 16 g (0.6 oz) an hour through the walls and door. Add the plants' 6 g an hour and a 12-hour day uses about 260 g (9 oz). The same 20 lb (9 kg) cylinder that lasts a day in a vented tent lasts about five weeks in that room.

Log-scale bar chart titled How long a 20 lb (9 kg) CO2 cylinder lasts at 1,200 ppm for one 4 x 4 ft tent's worth of flowering plants: about 1 day in a vented tent at 302 CFM, about 3 days at 112 CFM, and in a closed 10 x 10 x 8 ft room about 20 days at one air change an hour, 35 days at half and 55 days at a quarter.

If none of that describes your grow, the money is better spent elsewhere. In Westmoreland's chambers, doubling the light doubled yield at every CO2 level, room air included, and Rodriguez-Morrison and colleagues saw yield climb with light at room CO2 too. An even, trained canopy puts more of the light you already pay for onto bud sites, which our canopy science guide covers in detail. In a vented home tent, a flat canopy under a stronger light is the CO2 upgrade.

An even canopy of flowering cannabis plants spread under a trellis net inside a BudTrainer grow tent, frosty colas at a similar height across the tent and a yellow BudTrainer banner on the back wall.

Every Way to Add CO2, Compared

There are five common ways to put CO2 into a grow space. The useful way to compare them is not price but grams: how much CO2 each can actually deliver against what the space uses. A sealed 4 ft by 4 ft tent's plants use about 71 g (2.5 oz) in a 12-hour day. The same tent with its exhaust running at 112 CFM needs about 3,200 g (7 lb) a day to hold 1,200 ppm.

Log-scale bar chart titled What each CO2 source can deliver: a vented 4 x 4 ft tent at 112 CFM needs 3,180 g a day and a sealed one 71 g, against a 20 lb cylinder at 9,070 g, 1 lb of burned propane at 1,360 g, a mushroom bag at 11 g a day at most, yeast with 2 cups of sugar at 206 g per batch, and a cup of baking soda with vinegar at 115 g in a few minutes.
Source What it delivers Control Side effects Verdict
Cylinder, regulator and controller As much as you set; 9 kg (20 lb) per cylinder Exact, with a sensor None in the room The one to use in a sealed room or small room
Propane or natural gas burner About 3 lb of CO2 per lb of propane Good, on a controller Heat, water, a flame, carbon monoxide risk Large sealed rooms only, never a tent
Mushroom CO2 bag At most about 11 g a day, by mass None None Too little to measure in a vented tent
Yeast and sugar bottle About half the sugar's weight, over a week or two None Smell, fruit flies, refills A science project
Baking soda and vinegar About half the soda's weight, in minutes None A bucket of vinegar Gone before it does anything

Cylinder, regulator and controller

This is how most sealed home rooms do it, and how the research was done. A cylinder of compressed liquid CO2, the kind welding and beverage gas suppliers fill or swap, feeds a CO2 regulator. A grow-room regulator does three jobs: a gauge shows the cylinder pressure, a flow meter (the clear tube with a ball in it) sets how many liters a minute come out, and an electric solenoid valve opens and closes the flow when the controller tells it to. Common kits include ones from AC Infinity, Titan Controls and Vivosun.

Close-up of a brass CO2 regulator on an aluminum cylinder chained to a concrete wall: a round pressure gauge, an upright clear flow meter tube, a black solenoid valve with its power cord, and a gloved hand on the fittings, with a black grow tent behind.

The controller is the part that matters. It reads CO2 with an infrared (NDIR) sensor hung at canopy height and opens the solenoid only when the reading drops below your setpoint, so you get the level you asked for whatever the plants and the leaks are doing. A timer-only setup releases a fixed amount whether the room needs it or not, and is guesswork. Good controllers also have a light sensor or a daytime-only mode so they never inject in the dark.

A white CO2 controller with a dark screen and two knobs strapped to a pole inside a grow tent, its slim sensor hanging on a cord from the top of the tent above a canopy of flowering cannabis plants.

A 5 lb (2.3 kg) cylinder is easier to carry and lasts about nine days in the sealed room example above; a 20 lb (9 kg) cylinder lasts about five weeks. Many gas suppliers swap cylinders rather than refill yours, so ask before you buy one.

A small and a large plain aluminum CO2 cylinder, each with a brass valve and black handwheel, standing beside an orange 5-gallon bucket on a garage floor for scale.

Propane and natural gas burners

A CO2 generator burns propane or natural gas in a row of small jets inside a metal box that hangs above the canopy. Burning propane makes about three times its own weight in CO2: the US Energy Information Administration lists 12.68 lb of CO2 per gallon of propane, which works out to about 3 lb per lb.8 The catch is everything else the flame makes. Each pound of propane also releases about 1.6 lb (0.7 kg) of water and about 20,000 BTU of heat (our arithmetic from the chemistry), and any flame indoors can make carbon monoxide if it burns badly.

A large white grow room full of flowering cannabis plants in black fabric pots under many LED fixtures, with a gray open-fronted CO2 generator box holding a row of small brass burner jets hanging on chains above the canopy, a black gas hose running to the wall, and a mini-split air conditioner on the far wall.

Scaled to a tent, it is absurd. Holding a 4 ft by 4 ft tent at 1,200 ppm with its exhaust at 112 CFM would mean burning about 90 g (3 oz) of propane an hour, which puts about 1,100 W of heat into the tent, on top of the light. Burners are built for large sealed rooms where the air conditioning is sized for them. In a home tent or a small room, use a cylinder.

Mushroom CO2 bags (ExHale and similar)

A CO2 bag is a sealed grow bag of grain or similar substrate colonized by fungal mycelium. The fungus slowly digests the substrate and breathes out CO2 through a filter patch, the way any living thing does. ExHale is the best-known brand. Retailer listings say the bag raises CO2 by about 500 ppm on average in a space up to 4 ft by 4 ft or 128 cubic feet (3.6 m³), for up to six months, and list it at 4.5 lb (2 kg). We found no independent measurement of its output.

A clear plastic bag of pale grain substrate webbed with white mycelium, with a white filter patch near the top, hanging from the crossbar of a silver grow tent between flowering cannabis plants in black fabric pots.

The mass sets a ceiling. CO2 cannot come out of a bag that was not in it. If every gram of a 2 kg (4.5 lb) bag, water and plastic included, turned into CO2 over six months, which is chemically impossible, that would be about 11 g (0.4 oz) a day. Holding a 500 ppm rise in a vented 4 ft by 4 ft tent at 112 CFM takes about 2,000 g (4.4 lb) a day. Even in a sealed tent with no exhaust, the plants alone use about 71 g a day, so the bag's absolute best case is a seventh of their use. A mushroom bag does release CO2, and a bag in a tiny sealed cabinet may nudge the reading. In a vented tent, it cannot move the number you care about.

Yeast and sugar

Yeast fermenting sugar water makes alcohol and CO2. A sealed bottle with a tube through its cap is the classic do-it-yourself version. The chemistry caps it at about half the sugar's weight: 2 cups (400 g) of sugar makes at most about 200 g (7 oz) of CO2, over a week or two of fermentation, with the rate falling as the yeast tires. That is about 15 to 30 g (0.5 to 1 oz) a day, with no way to control when it comes out, a sour smell and a magnet for fruit flies. It is a fine science project and a poor way to feed a tent.

A capped 2-liter plastic bottle of cloudy yellow sugar water with a layer of foam from fermenting yeast, a thin clear tube rising from a hole in the cap, standing on the floor of a grow tent in front of cannabis plants in black fabric pots.

Baking soda and vinegar

Baking soda and vinegar give off about half the soda's weight as CO2, and they give it all at once. A cup (220 g) of baking soda makes about 115 g (4 oz) of CO2 in a few minutes. In a vented tent it is out the duct before it reaches the canopy, and in a sealed tent it is a single spike the plants use up in under an hour. It does not work as enrichment.

A white bowl of baking soda and vinegar fizzing into a mound of white foam on the floor of a grow tent beside a plain open box, flowering cannabis plants in black fabric pots behind.

CO2 Levels by Stage: How Much, and When

Hold CO2 only while the lights are on. In the dark, a plant does not photosynthesize; it only breathes, giving off CO2, so enrichment at night is wasted gas. Westmoreland did not enrich at night either.1

Two 24-hour clock dials titled Run CO2 only while the lights are on: veg at 18/6 with CO2 on from 6 am to midnight, and flower at 12/12 with CO2 on from 6 am to 6 pm; in the dark the plant gives CO2 off instead of taking it in.
Stage CO2 with lights on Why
Seedlings and clones Room air, about 425 ppm Small plants use little CO2; clones under a dome need fresh air and steady humidity, not CO2
Vegetative growth 800 to 1,200 ppm, sealed rooms only Faster growth, but veg is short and cheap; many growers save the gas for flower
Flowering 800 to 1,200 ppm, sealed rooms only This is where the measured 34 to 43% yield gain comes from; 90% of it is in hand at 1,200
Any stage, at night Off No photosynthesis in the dark

We stop at 1,200 ppm for three reasons. The trial shows diminishing returns above it. Every extra ppm leaks out of a real room at the same rate, so the gas cost climbs while the yield gain flattens. And it keeps a wide margin under the levels that start to affect people, below.

What changes when you run CO2

Illustration titled More CO2, narrower pores, warmer buds: two magnified circles of a leaf surface, on the left at room air with wide open pores and plenty of water vapor rising, on the right at 1,000 ppm and up with pores half closed and less vapor, noting buds ran about 2°C (3.6°F) warmer at 1,400 ppm.

Enriched plants close their stomata partway. Chandra and colleagues measured stomatal conductance down about 42% and transpiration down about 29% at 750 ppm.3 Less water leaving the leaf means three practical changes:

  • The buds run warmer. Water leaving the leaf is how a plant cools itself, so less of it means warmer tissue. In Westmoreland's trial, flowers were already 2 to 5°C (4 to 9°F) above the air, and enrichment added about 2°C (3.6°F) more.1 Watch the tops, as our foxtailing guide explains, and use our VPD guide's leaf-temperature check rather than the air thermometer alone.
  • The plants drink less per gram of growth. The pot dries back more slowly, so water by pot weight, as our watering guide describes, rather than by the calendar.
  • Calcium and magnesium can run short. These two move into the plant with the water, so less water carries less of them; Westmoreland measured both about 10% lower at 1,400 ppm. Watch for the early signs in our deficiencies guide and keep the feed strength right, as our nutrients guide covers.
A hand aiming a yellow and black handheld infrared thermometer with a blank screen at the top colas of a flat canopy of flowering cannabis plants under a trellis net and an LED bar light in a silver grow tent.

How to Set Up CO2 in a Sealed Grow Room, Step by Step

These steps assume the room passes every line of the "worth it" list above: closed while the lights are on, with an air conditioner and a dehumidifier carrying the heat and water.

Step 1 - Close the room and check it holds

Seal the room's door with weatherstripping, close or block any duct to the rest of the house, and set any tent inside it to exhaust into the room rather than out of it.

A fully zipped black grow tent in a small carpeted room, its silver exhaust duct leaving the tent top and ending open inside the same room, with a window air conditioner in the window and a white dehumidifier on the floor beside the tent.

Then test the room: release CO2 until a meter in the room reads about 1,000 ppm, turn the gas off, and watch how fast the reading falls with the lights off. A room that drops back to room air within an hour or two leaks too much to enrich; find the gaps first.

A gloved hand pressing a strip of black foam weatherstripping into the frame of a white door, with the purple glow of a grow light and a plant in a black fabric pot visible inside the room.

Step 2 - Chain the cylinder upright outside the canopy

Stand the cylinder upright in a corner of the room, away from the lights and any heater, and chain or strap it to the wall so it cannot fall. A falling cylinder can snap its valve. Keep it out of direct sun and anywhere it can get hot.

Step 3 - Fit the regulator and solenoid

Screw the regulator onto the cylinder valve by hand and then snug it with a wrench, with the sealing washer that came with it in place. Open the cylinder valve slowly, check the gauge, and brush soapy water over the joints; bubbles mean a leak. Set the flow meter to a low rate to start, about 1 to 2 liters a minute for a small room; the controller decides how long it flows.

A small brush dabbing soapy water on the brass joint where a CO2 regulator meets the cylinder, a cluster of soap bubbles forming on the threads beside the gauge and the clear flow meter tube.

Step 4 - Run the tube to just above the canopy

Run the tube from the solenoid up to the ceiling and out over the middle of the canopy, a little above the tops. CO2 is about one and a half times as heavy as air, so it settles downward: release it above the plants and let it fall into them, not at floor level where it pools under the pots.

Illustration titled Release CO2 above the canopy and keep the air moving: a tube releasing a pale blue cloud above a row of flowering cannabis plants that settles down into the leaves, and a fan on a pole at the right stirring it through the canopy.

Hang the end of the tube from hooks so it cannot drift down into the plants. Some growers run a length of tube with small holes punched along it across the canopy to spread the gas.

A thin black tube running along the ceiling of a small white grow room on hooks and hanging down to just above the middle of a flat canopy of flowering cannabis plants, an LED fixture beside it.

Step 5 - Hang the sensor at canopy height and wire the controller

Hang the controller's sensor at the height of the tops, in the middle of the canopy, out of the direct stream from the tube and away from the light. Plug the solenoid into the controller's outlet. Set the target to 1,000 to 1,200 ppm and turn on its daytime-only mode or light sensor so it never runs in the dark.

Step 6 - Lock out any exhaust while the CO2 runs

If the room still has an exhaust fan to the outside for emergencies or for the night, set the controller or a fan controller so the CO2 is off whenever that fan runs. Otherwise the two fight, and the fan wins.

Step 7 - Watch the plants for a week, then adjust

Watch the readings for the first few days: the room should reach the setpoint within minutes of lights-on and hold it. Check leaf and bud temperature at the tops, the pot weight before watering, and the newest leaves for the first signs of calcium or magnesium shortage. Adjust the room temperature and feed rather than the CO2.

CO2 Safety: What Is Dangerous, and How to Stay Below It

CO2 is not poisonous the way carbon monoxide is; we breathe it out all day. But at high concentrations it causes headaches, drowsiness and shortness of breath, and at very high concentrations it is dangerous. You cannot see it or smell it, so the only way to know the level is a meter.

Chart titled CO2 levels, from what plants need to what hurts people, one row per level from the top: 40,000 ppm immediately dangerous to life; 30,000 ppm the 15-minute limit; 5,000 ppm the 8-hour workplace limit; 1,400 ppm the highest level in the yield trial, +43% flower yield; 800 to 1,200 ppm our target in a sealed grow room, lights-on only; 425 ppm outdoor air, where a vented tent should stay; 50 ppm where the leaf stops taking in CO2.

The US exposure limits, from the NIOSH Pocket Guide to Chemical Hazards:9

  • 5,000 ppm averaged over an 8-hour working day: the OSHA permissible limit and the NIOSH recommended limit.
  • 30,000 ppm for any 15 minutes: the NIOSH short-term limit.
  • 40,000 ppm: immediately dangerous to life or health.

A grow room held at 1,200 ppm sits a long way below the first line. The danger is a fault: a stuck solenoid, a cracked tube or a cylinder left open overnight in a small closed room. CO2 is heavier than air, so it collects low, in a basement or at the floor of a closet. The rules that keep you safe:

  • Put a CO2 monitor with an alarm in the room, at head height, separate from the controller's sensor. If the controller fails, the second meter is the one that tells you.
  • Burners only with a carbon monoxide alarm in the same room, and only in rooms built for them.
  • Never enrich a bedroom, a closet in a living space, or any room someone sleeps in.
  • Close the cylinder valve at the end of the day if your controller does not cut the flow reliably, and whenever you leave the grow for more than a day.
  • If you feel a headache or short of breath in the room, leave, open the door, and check the meter before you go back in.
  • Keep children and pets out of an enriched room.
A white CO2 monitor with a blank screen and a round white carbon monoxide alarm mounted at head height on a wall beside a black grow tent, above an aluminum CO2 cylinder chained to the wall with its regulator, flow meter and solenoid fitted.

Common CO2 Mistakes

Adding CO2 to a tent with the exhaust running

The most common one, and the most expensive: 98 to 99 percent of the gas goes out the duct. If you cannot close the space, do not buy the cylinder.

Starving the tent with a weak exhaust

The quiet mistake. A tent with a tiny fan, a fan on a long off-cycle, or every port zipped shut can run 100 ppm or more below the room for the whole day, which costs more yield than CO2 could ever add.

The lower corner of a black grow tent in a basement with a round port near the floor opened and its sleeve rolled back, a mesh screen across it, young cannabis plants visible inside through the open door flap.

Using a timer instead of a sensor

A timer releases the same amount whatever the room is doing. Some days that is too little, and on a day the plants use less it is too much. A controller reads the air.

Expecting CO2 to raise potency

It raised yield in the trials and did not consistently change cannabinoid concentration. Buy it for grams, not for percent THC.

Adding CO2 before the basics are right

CO2 multiplies a good grow. An uneven canopy that topping and low-stress training would have leveled, a weak light, the wrong pH or a pest problem will still be there at 1,200 ppm, and it will still be the thing limiting your harvest.

Frequently Asked Questions

Does a grow tent need CO2?

No. A vented grow tent needs fresh air, not added CO2. With an exhaust fan running, the tent stays close to room air, about 425 ppm, which grows excellent cannabis. Added CO2 only pays in a space that is closed to outside air while the lights are on, with an air conditioner and dehumidifier doing the exhaust fan's job. In a vented tent most of it leaves through the duct within a minute.

What is the ideal CO2 level for a grow tent?

In a vented tent, the goal is room air, about 425 ppm, and never below it; a tent that falls 100 ppm under room air loses more yield than enrichment adds. In a sealed room, hold 800 to 1,200 ppm with the lights on. In growth-chamber trials, 800 ppm gave about 70% of the full yield gain and 1,200 ppm gave 90% of it.

How much does CO2 increase cannabis yield?

In Mitchell Westmoreland's 2024 Utah State trials, raising CO2 from about 420 to 1,400 ppm raised flower yield by 43%, and 460 to 1,200 ppm raised it 34%. The gain held at moderate and very bright light and in both cultivars tested. Cannabinoid concentration did not change consistently. It was measured in sealed growth chambers, with everything else ideal.

Is CO2 worth it in a 4x4 grow tent?

Not if the tent vents outside the room. Holding a 4 ft by 4 ft tent at 1,200 ppm with its exhaust at 112 CFM takes about 265 g of CO2 an hour, so a 20 lb cylinder lasts about 34 hours of lights-on. It is worth it if the tent sits in a closed room with its own air conditioner and dehumidifier, exhausting into that room.

Do ExHale CO2 bags work?

Not in a vented tent. A mushroom bag does release CO2, but its mass caps the output: even if all 4.5 lb (2 kg) of the bag turned into CO2 over six months, which is impossible, that is about 11 g a day. A vented 4 ft by 4 ft tent loses around 3,000 g a day at 1,200 ppm, and even sealed plants use about 71 g.

Does baking soda and vinegar make CO2 for plants?

Yes, but all at once and not much. A cup (220 g) of baking soda gives off about 115 g of CO2 within minutes. In a vented tent it leaves through the exhaust before it does anything, and in a sealed tent it is a brief spike the plants use within an hour. It cannot hold a level, so it does not work as enrichment.

How long does a 20 lb CO2 tank last in a grow room?

It depends on how much air leaves the room. In a vented 4 ft by 4 ft tent held at 1,200 ppm it lasts 13 to 34 hours of lights-on. In a closed 10 ft by 10 ft room with one tent's worth of plants, leaking half an air change an hour, it lasts about five weeks of 12-hour days. Tightness, not plant count, sets the answer.

Should I turn off my exhaust fan when using CO2?

Only if something else removes the heat and humidity, an air conditioner and a dehumidifier sized for the room. Otherwise the tent overheats and the humidity climbs within minutes of lights-on. A controller can lock the CO2 out whenever the exhaust runs, which wastes less gas, but in most LED tents the fan runs too often for that to hold a level.

Should CO2 run at night?

No. Plants take in CO2 only while photosynthesizing, which needs light. In the dark they give off CO2 instead. Run enrichment only during lights-on, using a controller with a daytime mode or a light sensor; the yield research did not enrich at night.

Do I need CO2 if my light is under 1,000 PPFD?

Light is not what decides it. In Westmoreland's trials the relative yield gain from CO2 was the same at 750 µmol/m²/s as at 1,500. What decides it is whether the space can hold the CO2. And at room CO2, yield keeps rising with more light, so in a vented tent a stronger light and an even canopy are the better upgrade.

Is CO2 dangerous in a grow room?

At 1,200 ppm, no. The US workplace limit is 5,000 ppm averaged over 8 hours, 30,000 ppm for 15 minutes, and 40,000 ppm is immediately dangerous. The risk is a fault, such as a stuck valve or an open cylinder in a small closed room. Keep a separate CO2 alarm at head height, and never enrich a room anyone sleeps in.

Does CO2 increase THC?

Not reliably. In the only controlled cannabis yield trial we found, enrichment to 1,400 ppm raised flower yield by up to 43%, but cannabinoid concentration fell in one treatment and was unchanged in the rest. More grams of flower means more total cannabinoids per plant, but not a higher percentage in each bud.

Do plants need more water or nutrients with CO2?

Less water per gram of growth, and a closer eye on feed. CO2 partly closes the leaf pores, so plants transpire less: Chandra and colleagues measured transpiration down about 29% at 750 ppm. Calcium and magnesium travel in with the water, and Westmoreland measured both about 10% lower at 1,400 ppm, so watch for early shortages and keep feed strength right.

Where to Go From Here

For most home grows the CO2 work is done by the exhaust fan, so the next step is making sure it is sized and running properly, which our indoor growing guide covers alongside the light, the tent and the timer. The bigger yield lever in a vented tent is the light you already have: our light schedule guide shows how hours and intensity add up to a daily dose, and our flowering stage guide sets the light and climate for the weeks that make the harvest. Training is how you spread that light: LST clips like the BudClips hold branches out into an even canopy in veg, and fabric pots like the BudPots give the roots the air and the steady dry-back that every one of these upgrades depends on.

References

  1. Westmoreland, F. M. (2024). Environmental physiology of medical cannabis [Doctoral dissertation, Utah State University; committee chair B. Bugbee]. Chapter 6, Exploring the limits of crop productivity from photosynthesis to yield: response to elevated CO2. https://doi.org/10.26076/1d25-9d05
  2. NOAA Global Monitoring Laboratory. Mauna Loa CO2 annual mean data (2024: 424.61 ppm; 2025, preliminary: 427.35 ppm). https://gml.noaa.gov/ccgg/trends/data.html
  3. Chandra, S., Lata, H., Khan, I. A., & ElSohly, M. A. (2008). Photosynthetic response of Cannabis sativa L. to variations in photosynthetic photon flux densities, temperature and CO2 conditions. Physiology and Molecular Biology of Plants, 14(4), 299-306. https://doi.org/10.1007/s12298-008-0027-x
  4. Chandra, S., Lata, H., Khan, I. A., & ElSohly, M. A. (2011). Photosynthetic response of Cannabis sativa L., an important medicinal plant, to elevated levels of CO2. Physiology and Molecular Biology of Plants, 17(3), 291-295. https://doi.org/10.1007/s12298-011-0066-6
  5. Ainsworth, E. A., & Rogers, A. (2007). The response of photosynthesis and stomatal conductance to rising [CO2]: mechanisms and environmental interactions. Plant, Cell & Environment, 30(3), 258-270. https://doi.org/10.1111/j.1365-3040.2007.01641.x
  6. Kimball, B. A. (1983). Carbon dioxide and agricultural yield: an assemblage and analysis of 430 prior observations. Agronomy Journal, 75(5), 779-788. https://doi.org/10.2134/agronj1983.00021962007500050014x
  7. Rodriguez-Morrison, V., Llewellyn, D., & Zheng, Y. (2021). Cannabis yield, potency, and leaf photosynthesis respond differently to increasing light levels in an indoor environment. Frontiers in Plant Science, 12, 646020. https://doi.org/10.3389/fpls.2021.646020
  8. US Energy Information Administration. Carbon dioxide emissions coefficients by fuel. https://www.eia.gov/environment/emissions/co2_vol_mass.php
  9. National Institute for Occupational Safety and Health. NIOSH Pocket Guide to Chemical Hazards: carbon dioxide. Centers for Disease Control and Prevention. https://www.cdc.gov/niosh/npg/npgd0103.html
Henrique Dias, CEO and co-founder of BudTrainer.

About the Author

Henrique Dias is the CEO and co-founder of BudTrainer. He is a mechanical engineer who also holds a graduate certificate in Commercial Cannabis Production from Niagara College in Ontario, Canada - the first college-level cannabis cultivation program in North America. Before founding BudTrainer, Henrique worked with Health Canada licensed cannabis producers, where he gained hands-on experience in cultivation, processing, and post-harvest management of cannabis at commercial scale. He started BudTrainer to bring that same level of craft to home growers through better-designed gardening tools and clear, science-backed education.

DISCLAIMER: Everything taught and sold by BudTrainer® is to be used strictly for legal purposes. We do not endorse the production of illegal substances and it is your duty to ensure that you are complying with the law. The words "hemp," "cannabis," "weed," and "marijuana" are used interchangeably to refer to the same plant (legal hemp with less than 0.3% THC) for the purposes of this lesson.

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